Hotspot

rps2

LSC · 15319-16029 · 711 bp

Back to Bennettiodendron

Pi 0

MarkerSeek score 26.10

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	GAAATGCTGGCCTACTTCGA	AAGCGATCTGTTACCGAACC	50.0	50.0	58.0	58.0	1635-1640 bp	1.0	80.0
rps2_p2	AGAAATGCTGGCCTACTTCG	AAGCGATCTGTTACCGAACC	50.0	50.0	58.0	58.0	1636-1641 bp	1.0	79.9
rps2_p3	GAAATGCTGGCCTACTTCGA	TCGCGTTTTAGCAAAAGCTG	50.0	45.0	58.0	58.0	1541-1546 bp	1.0	79.8
rps2_p4	GAAATGCTGGCCTACTTCGA	TGCTCGCGTTTTAGCAAAAG	50.0	45.0	58.0	58.0	1544-1549 bp	1.0	79.8
rps2_p5	AGAAATGCTGGCCTACTTCG	TCGCGTTTTAGCAAAAGCTG	50.0	45.0	58.0	58.0	1542-1547 bp	1.0	79.7