Hotspot

petA

LSC · 63252-64214 · 963 bp

Back to Bennettiodendron

Pi 0.00208

MarkerSeek score 59.90

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 2
pi: 0.00208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AGGGAACTATCCTAGCGACC	AGAACTTGATATTGATAAATTCGCA	55.0	28.0	58.0	55.5	1054 bp	1.0	40.2
petA_p2	GGGAACTATCCTAGCGACCT	AGAACTTGATATTGATAAATTCGCA	55.0	28.0	58.0	55.5	1053 bp	1.0	40.2
petA_p3	CAGCATCGTGGATAGGGAAC	AGAACTTGATATTGATAAATTCGCA	55.0	28.0	58.1	55.5	1067 bp	1.0	40.1
petA_p4	AGGGAACTATCCTAGCGACC	TCATACAGAATTTCCAACAAAA	55.0	27.3	58.0	52.2	1115 bp	1.0	40.1
petA_p5	GGGAACTATCCTAGCGACCT	TCATACAGAATTTCCAACAAAA	55.0	27.3	58.0	52.2	1114 bp	1.0	40.1