Hotspot

ndhK

LSC · 50162-50839 · 678 bp

Back to Bennettiodendron

Pi 0.00442

MarkerSeek score 61.30

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 3
pi: 0.00442
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TTCTCTCTCCCCCATTTTGA	TGGCGAAAAGGAGCATTAGA	45.0	45.0	55.7	57.2	868 bp	1.0	55.8
ndhK_p2	TCTGTACAAAGGAGTCCTTCC	TGGCGAAAAGGAGCATTAGA	47.6	45.0	56.7	57.2	788 bp	1.0	55.4
ndhK_p3	TCTCTCCCCCATTTTGATTTGT	TGGCGAAAAGGAGCATTAGA	40.9	45.0	57.6	57.2	865 bp	1.0	54.8
ndhK_p4	TTTCTCTCTCCCCCATTTTGA	TGGCGAAAAGGAGCATTAGA	42.9	45.0	56.5	57.2	869 bp	1.0	54.3
ndhK_p5	TTCTCTCTCCCCCATTTTGA	TATGCATGGCGAAAAGGAGC	45.0	50.0	55.7	59.0	874 bp	1.0	54.2