Hotspot

ccsA

SSC · 117293-118258 · 966 bp

Back to Bennettiodendron

Pi 0.00414

MarkerSeek score 46.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 4
pi: 0.00414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	AGCTTCTCTTGCATTACCGG	50.0	50.0	58.0	58.0	1770 bp	1.0	80.0
ccsA_p2	TGAAATCGGTAGACACGCTG	AGCTTCTCTTGCATTACCGG	50.0	50.0	58.0	58.0	1769 bp	1.0	80.0
ccsA_p3	GTGAAATCGGTAGACACGCT	TATCTTGACGAAATGGGCGG	50.0	50.0	58.0	58.1	1841 bp	1.0	79.8
ccsA_p4	TGAAATCGGTAGACACGCTG	TATCTTGACGAAATGGGCGG	50.0	50.0	58.0	58.1	1840 bp	1.0	79.8
ccsA_p5	TAGACACGCTGCTCTTAGGA	AGCTTCTCTTGCATTACCGG	50.0	50.0	57.9	58.0	1760 bp	1.0	79.0