Hotspot

psbE-petL

LSC · 66065-67500 · 1436 bp

Back to Bennettiodendron

Pi 0.00173
MarkerSeek score 55.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1436
variable_sites
2
pi
0.00173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC AGTCGTATCTTGCTCAGACT 50.0 45.0 57.8 55.7 1293-1552 bp 1.0 64.5
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC AGTCGTATCTTGCTCAGACT 55.0 45.0 58.2 55.7 1288-1547 bp 1.0 64.2
psbE-petL_p3 TCTCCTGTGCTTCCAGACAT AGTCGTATCTTGCTCAGACT 50.0 45.0 58.4 55.7 1286-1545 bp 1.0 63.0
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC AAGTCGTATCTTGCTCAGACT 50.0 42.9 57.8 56.5 1294-1553 bp 1.0 62.8
psbE-petL_p5 GTTCTCCTGTGCTTCCAGAC AAGTCGTATCTTGCTCAGACT 55.0 42.9 58.2 56.5 1289-1548 bp 1.0 62.5