Hotspot

rps2-rpoC2

LSC · 16030-16277 · 248 bp

Back to Bennettiodendron

Pi 0.0161

MarkerSeek score 61.80

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 248
variable_sites: 4
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AGGTGCCATTTTCGGATTCC	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.5	57.8	464-469 bp	1.0	74.5
rps2-rpoC2_p2	AGGTGCCATTTTCGGATTCC	TCGAGGGGGAAATGAGAGAT	50.0	50.0	58.5	57.5	463-468 bp	1.0	72.7
rps2-rpoC2_p3	GGTGCCATTTTCGGATTCCA	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.8	57.8	463-468 bp	1.0	72.2
rps2-rpoC2_p4	GGTGCCATTTTCGGATTCCA	TCGAGGGGGAAATGAGAGAT	50.0	50.0	58.8	57.5	462-467 bp	1.0	70.3
rps2-rpoC2_p5	GTGCCATTTTCGGATTCCAT	TTCGAGGGGGAAATGAGAGA	45.0	50.0	56.7	57.8	462-467 bp	1.0	68.7