Hotspot

atpH-atpI

LSC · 13388-14356 · 969 bp

Back to Bennettiodendron

Pi 0.0031

MarkerSeek score 56.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 969
variable_sites: 3
pi: 0.0031
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GTGAATCCATGGAGGGACAC	45.0	55.0	58.0	58.0	1027-1239 bp	1.0	79.6
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	GTGAATCCATGGAGGGACAC	45.0	55.0	57.9	58.0	1019-1231 bp	1.0	79.4
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1056-1268 bp	1.0	78.8
atpH-atpI_p4	GTCCAATAGAAGCAAGCCCA	GTGAATCCATGGAGGGACAC	50.0	55.0	57.9	58.0	1065-1277 bp	1.0	78.8
atpH-atpI_p5	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1048-1260 bp	1.0	78.6