Hotspot

atpA-atpF

LSC · 11280-11340 · 61 bp

Back to Bennettiodendron

Pi 0.0339

MarkerSeek score 75.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 2
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CACTTGAAGTACGGTCCCAG	TGTTTGGGGCGATGAAAGAA	55.0	45.0	57.9	58.0	196-198 bp	1.0	80.9
atpA-atpF_p2	TACTAATCTCGTCGGCTCGA	TGTTTGGGGCGATGAAAGAA	50.0	45.0	57.8	58.0	122-124 bp	1.0	80.7
atpA-atpF_p3	CACTTGAAGTACGGTCCCAG	ATGTTTGGGGCGATGAAAGA	55.0	45.0	57.9	57.8	197-199 bp	1.0	79.3
atpA-atpF_p4	ACTTGAAGTACGGTCCCAGT	TGTTTGGGGCGATGAAAGAA	50.0	45.0	58.3	58.0	195-197 bp	1.0	79.2
atpA-atpF_p5	CACTTGAAGTACGGTCCCAG	CATGTTTGGGGCGATGAAAG	55.0	50.0	57.9	57.7	198-200 bp	1.0	78.7