Hotspot

trnG-UCC-trnR-UCU

LSC · 9297-9517 · 221 bp

Back to Bennettiodendron

Pi 0.00461

MarkerSeek score 52.00

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 221
variable_sites: 1
pi: 0.00461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	TTCCAAGCTAATGATGCGGG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.3	56.9	293-297 bp	1.0	62.4
trnG-UCC-trnR-UCU_p2	TTCCAAGCTAATGATGCGGG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.3	57.9	299-303 bp	1.0	61.7
trnG-UCC-trnR-UCU_p3	GCTAATGATGCGGGTTCGAT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.5	56.9	287-291 bp	1.0	61.1
trnG-UCC-trnR-UCU_p4	GCTAATGATGCGGGTTCGAT	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.5	57.9	293-297 bp	1.0	60.4
trnG-UCC-trnR-UCU_p5	CCAAGCTAATGATGCGGGTT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.6	56.9	291-295 bp	1.0	60.1