primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACTCCCGAAAGATAAGTGGG	TGGGTTGCTAACTCAATGGT	20	20	50.0	45.0	56.6	57.0	0.0	0.0	2.343838	3558	4316	882-883	1.000000	61.4
matK-trnK-UUU_p2	matK-trnK-UUU	2	CTCCCGAAAGATAAGTGGGT	TGGGTTGCTAACTCAATGGT	20	20	50.0	45.0	56.6	57.0	0.0	0.0	2.343838	3558	4316	881-882	1.000000	61.4
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACTCCCGAAAGATAAGTGGG	TCAATGGTAGAGTACTCGGCT	20	21	50.0	47.6	56.6	58.0	0.0	17.1	2.388320	3558	4316	870-871	1.000000	61.1
matK-trnK-UUU_p4	matK-trnK-UUU	4	CTCCCGAAAGATAAGTGGGT	TCAATGGTAGAGTACTCGGCT	20	21	50.0	47.6	56.6	58.0	0.0	17.1	2.388320	3558	4316	869-870	1.000000	61.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACTCCCGAAAGATAAGTGGGT	TGGGTTGCTAACTCAATGGT	21	20	47.6	45.0	58.5	57.0	0.0	0.0	2.421832	3558	4316	882-883	1.000000	60.8
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	TTCCAAGCTAATGATGCGGG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.3	56.9	0.0	0.0	2.372466	9297	9517	293-297	1.000000	62.4
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	TTCCAAGCTAATGATGCGGG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.3	57.9	0.0	0.0	2.455793	9297	9517	299-303	1.000000	61.7
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	GCTAATGATGCGGGTTCGAT	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.5	56.9	0.0	0.0	2.538678	9297	9517	287-291	1.000000	61.1
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	GCTAATGATGCGGGTTCGAT	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.5	57.9	0.0	0.0	2.622006	9297	9517	293-297	1.000000	60.4
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCAAGCTAATGATGCGGGTT	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.6	56.9	0.0	0.0	2.659015	9297	9517	291-295	1.000000	60.1
atpA-atpF_p1	atpA-atpF	1	CACTTGAAGTACGGTCCCAG	TGTTTGGGGCGATGAAAGAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.091993	11280	11340	196-198	1.000000	80.9
atpA-atpF_p2	atpA-atpF	2	TACTAATCTCGTCGGCTCGA	TGTTTGGGGCGATGAAAGAA	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.241382	11280	11340	122-124	1.000000	80.7
atpA-atpF_p3	atpA-atpF	3	CACTTGAAGTACGGTCCCAG	ATGTTTGGGGCGATGAAAGA	20	20	55.0	45.0	57.9	57.8	0.0	0.0	0.294662	11280	11340	197-199	1.000000	79.3
atpA-atpF_p4	atpA-atpF	4	ACTTGAAGTACGGTCCCAGT	TGTTTGGGGCGATGAAAGAA	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.298409	11280	11340	195-197	1.000000	79.2
atpA-atpF_p5	atpA-atpF	5	CACTTGAAGTACGGTCCCAG	CATGTTTGGGGCGATGAAAG	20	20	55.0	50.0	57.9	57.7	0.0	0.0	0.362987	11280	11340	198-200	1.000000	78.7
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	GTGAATCCATGGAGGGACAC	20	20	45.0	55.0	58.0	58.0	1.4	21.3	0.082510	13388	14356	1027-1239	1.000000	79.6
atpH-atpI_p2	atpH-atpI	2	AGCGGCAGAAATCAATGGAT	GTGAATCCATGGAGGGACAC	20	20	45.0	55.0	57.9	58.0	1.4	21.3	0.107093	13388	14356	1019-1231	1.000000	79.4
atpH-atpI_p3	atpH-atpI	3	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	13388	14356	1056-1268	1.000000	78.8
atpH-atpI_p4	atpH-atpI	4	GTCCAATAGAAGCAAGCCCA	GTGAATCCATGGAGGGACAC	20	20	50.0	55.0	57.9	58.0	0.0	21.3	0.172893	13388	14356	1065-1277	1.000000	78.8
atpH-atpI_p5	atpH-atpI	5	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	57.9	57.9	1.4	0.0	0.195711	13388	14356	1048-1260	1.000000	78.6
rps2_p1	rps2	1	GAAATGCTGGCCTACTTCGA	AAGCGATCTGTTACCGAACC	20	20	50.0	50.0	58.0	58.0	3.3	0.0	0.030146	15319	16029	1635-1640	1.000000	80.0
rps2_p2	rps2	2	AGAAATGCTGGCCTACTTCG	AAGCGATCTGTTACCGAACC	20	20	50.0	50.0	58.0	58.0	3.3	0.0	0.035809	15319	16029	1636-1641	1.000000	79.9
rps2_p3	rps2	3	GAAATGCTGGCCTACTTCGA	TCGCGTTTTAGCAAAAGCTG	20	20	50.0	45.0	58.0	58.0	3.3	5.8	0.055212	15319	16029	1541-1546	1.000000	79.8
rps2_p4	rps2	4	GAAATGCTGGCCTACTTCGA	TGCTCGCGTTTTAGCAAAAG	20	20	50.0	45.0	58.0	58.0	3.3	16.7	0.055212	15319	16029	1544-1549	1.000000	79.8
rps2_p5	rps2	5	AGAAATGCTGGCCTACTTCG	TCGCGTTTTAGCAAAAGCTG	20	20	50.0	45.0	58.0	58.0	3.3	5.8	0.060875	15319	16029	1542-1547	1.000000	79.7
rps2-rpoC2_p1	rps2-rpoC2	1	AGGTGCCATTTTCGGATTCC	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.5	57.8	0.0	0.0	0.773369	16030	16277	464-469	1.000000	74.5
rps2-rpoC2_p2	rps2-rpoC2	2	AGGTGCCATTTTCGGATTCC	TCGAGGGGGAAATGAGAGAT	20	20	50.0	50.0	58.5	57.5	0.0	0.0	1.004542	16030	16277	463-468	1.000000	72.7
rps2-rpoC2_p3	rps2-rpoC2	3	GGTGCCATTTTCGGATTCCA	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.8	57.8	3.1	0.0	1.065913	16030	16277	463-468	1.000000	72.2
rps2-rpoC2_p4	rps2-rpoC2	4	GGTGCCATTTTCGGATTCCA	TCGAGGGGGAAATGAGAGAT	20	20	50.0	50.0	58.8	57.5	3.1	0.0	1.297085	16030	16277	462-467	1.000000	70.3
rps2-rpoC2_p5	rps2-rpoC2	5	GTGCCATTTTCGGATTCCAT	TTCGAGGGGGAAATGAGAGA	20	20	45.0	50.0	56.7	57.8	3.1	0.0	1.502831	16030	16277	462-467	1.000000	68.7
trnT-GGU_p1	trnT-GGU	1	ATCCGCATGCTGGATCATTT	CCGGGGGAGGTTTATGAAAA	20	20	45.0	50.0	58.0	57.5	27.6	0.0	0.526877	32109	32180	138-138	1.000000	76.4
trnT-GGU_p2	trnT-GGU	2	ATCCGCATGCTGGATCATTT	ATAGAAATACTCCCGGGGGA	20	20	45.0	50.0	58.0	56.9	27.6	32.3	1.118480	32109	32180	150-150	1.000000	71.6
trnT-GGU_p3	trnT-GGU	3	ATCCGCATGCTGGATCATTT	CCGGGGGAGGTTTATGAAAAA	20	21	45.0	47.6	58.0	58.2	27.6	0.0	1.198715	32109	32180	138-138	1.000000	71.0
trnT-GGU_p4	trnT-GGU	4	GCATGCTGGATCATTTCGGA	CCGGGGGAGGTTTATGAAAA	20	20	50.0	50.0	58.7	57.5	11.4	0.0	1.206139	32109	32180	134-134	1.000000	70.9
trnT-GGU_p5	trnT-GGU	5	TCCGCATGCTGGATCATTTC	CCGGGGGAGGTTTATGAAAA	20	20	50.0	50.0	58.7	57.5	22.0	0.0	1.206139	32109	32180	137-137	1.000000	70.9
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	TCTACCTGCTGAGCTATCCC	20	20	50.0	55.0	57.5	58.0	0.0	5.8	0.476915	48400	48728	383-383	1.000000	78.1
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	48400	48728	419-419	1.000000	77.5
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCTG	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.610708	48400	48728	395-395	1.000000	76.9
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	20	20	50.0	50.0	57.5	57.5	0.0	20.1	0.931776	48400	48728	416-416	1.000000	74.3
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	TTCAAGTCCCTCTATCCCCA	TCTACCTGCTGAGCTATCCC	20	20	50.0	55.0	57.0	58.0	0.0	5.8	0.964041	48400	48728	372-372	1.000000	74.2
ndhK_p1	ndhK	1	TTCTCTCTCCCCCATTTTGA	TGGCGAAAAGGAGCATTAGA	20	20	45.0	45.0	55.7	57.2	0.0	0.0	3.057150	50162	50839	868-868	1.000000	55.8
ndhK_p2	ndhK	2	TCTGTACAAAGGAGTCCTTCC	TGGCGAAAAGGAGCATTAGA	21	20	47.6	45.0	56.7	57.2	24.8	0.0	3.109834	50162	50839	788-788	1.000000	55.4
ndhK_p3	ndhK	3	TCTCTCCCCCATTTTGATTTGT	TGGCGAAAAGGAGCATTAGA	22	20	40.9	45.0	57.6	57.2	0.0	0.0	3.182371	50162	50839	865-865	1.000000	54.8
ndhK_p4	ndhK	4	TTTCTCTCTCCCCCATTTTGA	TGGCGAAAAGGAGCATTAGA	21	20	42.9	45.0	56.5	57.2	0.0	0.0	3.251268	50162	50839	869-869	1.000000	54.3
ndhK_p5	ndhK	5	TTCTCTCTCCCCCATTTTGA	TATGCATGGCGAAAAGGAGC	20	20	45.0	50.0	55.7	59.0	0.0	10.2	3.253124	50162	50839	874-874	1.000000	54.2
trnM-CAU_p1	trnM-CAU	1	TGTGACGGGTTCCATTTCTT	AGATACCATGGATTCGGATATCT	20	23	45.0	39.1	57.4	56.2	0.0	21.5	5.464743	53437	53508	145-145	1.000000	40.6
trnM-CAU_p2	trnM-CAU	2	CGATGTGACGGGTTCCATTT	AGATACCATGGATTCGGATATCT	20	23	50.0	39.1	58.6	56.2	0.0	21.5	5.366227	53437	53508	148-148	1.000000	40.5
trnM-CAU_p3	trnM-CAU	3	TAATCCATCGATGTGACGGG	AGATACCATGGATTCGGATATCT	20	23	50.0	39.1	57.2	56.2	23.3	21.5	5.627570	53437	53508	156-156	1.000000	40.5
trnM-CAU_p4	trnM-CAU	4	CGATGTGACGGGTTCCATTT	TTAGATACCATGGATTCGGA	20	20	50.0	40.0	58.6	52.9	0.0	21.5	5.651948	53437	53508	150-150	1.000000	40.5
trnM-CAU_p5	trnM-CAU	5	ATGTGACGGGTTCCATTTCT	AGATACCATGGATTCGGATATCT	20	23	45.0	39.1	57.1	56.2	0.0	21.5	5.694314	53437	53508	146-146	1.000000	40.5
rbcL-accD_p1	rbcL-accD	1	GCAAATGGAGCCCTGAACTA	CGGGTCTTCACTTACACTGG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209057	57914	58583	873-873	1.000000	78.5
rbcL-accD_p2	rbcL-accD	2	TAGCAAATGGAGCCCTGAAC	CGGGTCTTCACTTACACTGG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209057	57914	58583	875-875	1.000000	78.5
rbcL-accD_p3	rbcL-accD	3	AATGGAGCCCTGAACTAGCT	CGGGTCTTCACTTACACTGG	20	20	50.0	55.0	58.4	57.9	0.0	0.0	0.490872	57914	58583	870-870	1.000000	76.3
rbcL-accD_p4	rbcL-accD	4	CTAGCAAATGGAGCCCTGAA	CGGGTCTTCACTTACACTGG	20	20	50.0	55.0	57.6	57.9	0.0	0.0	0.505429	57914	58583	876-876	1.000000	76.1
rbcL-accD_p5	rbcL-accD	5	AAATGGAGCCCTGAACTAGC	CGGGTCTTCACTTACACTGG	20	20	50.0	55.0	57.6	57.9	0.0	0.0	0.507128	57914	58583	871-871	1.000000	76.1
petA_p1	petA	1	AGGGAACTATCCTAGCGACC	AGAACTTGATATTGATAAATTCGCA	20	25	55.0	28.0	58.0	55.5	0.0	0.0	7.540265	63252	64214	1054-1054	1.000000	40.2
petA_p2	petA	2	GGGAACTATCCTAGCGACCT	AGAACTTGATATTGATAAATTCGCA	20	25	55.0	28.0	58.0	55.5	0.0	0.0	7.540265	63252	64214	1053-1053	1.000000	40.2
petA_p3	petA	3	CAGCATCGTGGATAGGGAAC	AGAACTTGATATTGATAAATTCGCA	20	25	55.0	28.0	58.1	55.5	0.0	0.0	7.667407	63252	64214	1067-1067	1.000000	40.1
petA_p4	petA	4	AGGGAACTATCCTAGCGACC	TCATACAGAATTTCCAACAAAA	20	22	55.0	27.3	58.0	52.2	0.0	0.0	7.802807	63252	64214	1115-1115	1.000000	40.1
petA_p5	petA	5	GGGAACTATCCTAGCGACCT	TCATACAGAATTTCCAACAAAA	20	22	55.0	27.3	58.0	52.2	0.0	0.0	7.802807	63252	64214	1114-1114	1.000000	40.1
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACT	20	20	50.0	45.0	57.8	55.7	24.9	0.0	2.445748	66065	67500	1293-1552	1.000000	64.5
psbE-petL_p2	psbE-petL	2	GTTCTCCTGTGCTTCCAGAC	AGTCGTATCTTGCTCAGACT	20	20	55.0	45.0	58.2	55.7	0.9	0.0	2.487336	66065	67500	1288-1547	1.000000	64.2
psbE-petL_p3	psbE-petL	3	TCTCCTGTGCTTCCAGACAT	AGTCGTATCTTGCTCAGACT	20	20	50.0	45.0	58.4	55.7	0.9	0.0	2.640084	66065	67500	1286-1545	1.000000	63.0
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	AAGTCGTATCTTGCTCAGACT	20	21	50.0	42.9	57.8	56.5	24.9	0.0	2.662047	66065	67500	1294-1553	1.000000	62.8
psbE-petL_p5	psbE-petL	5	GTTCTCCTGTGCTTCCAGAC	AAGTCGTATCTTGCTCAGACT	20	21	55.0	42.9	58.2	56.5	0.9	0.0	2.703635	66065	67500	1289-1548	1.000000	62.5
psbT-psbN_p1	psbT-psbN	1	TCGAGAACCGCCTAAAGTTC	GATCCATTCGAGGAACACGG	20	20	50.0	55.0	57.6	58.4	0.0	0.0	0.787020	76273	76332	127-127	1.000000	75.0
psbT-psbN_p2	psbT-psbN	2	GAGAACCGCCTAAAGTTCCA	GATCCATTCGAGGAACACGG	20	20	50.0	55.0	57.5	58.4	0.0	0.0	0.895647	76273	76332	125-125	1.000000	74.1
psbT-psbN_p3	psbT-psbN	3	TCGAGAACCGCCTAAAGTTC	GGCAACCCTCTCAACAACTA	20	20	50.0	50.0	57.6	57.4	0.0	0.0	0.920417	76273	76332	150-150	1.000000	73.7
psbT-psbN_p4	psbT-psbN	4	TCGAGAACCGCCTAAAGTTC	AGATCCATTCGAGGAACACG	20	20	50.0	50.0	57.6	57.4	0.0	0.0	0.945197	76273	76332	128-128	1.000000	73.7
psbT-psbN_p5	psbT-psbN	5	GAGAACCGCCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	20	20	50.0	50.0	57.5	57.4	0.0	0.0	1.029044	76273	76332	148-148	1.000000	72.8
ycf1_p1	ycf1	1	TGTCGTTTCTAGTCTATCTGTTTCT	TCTTTGCAGGAGAAGGCATC	25	20	36.0	50.0	57.5	57.9	0.0	0.0	5.569925	112427	114235	2017-2075	1.000000	40.2
ycf1_p2	ycf1	2	TGTCGTTTCTAGTCTATCTGT	TCTTTGCAGGAGAAGGCATC	21	20	38.1	50.0	53.5	57.9	0.0	0.0	5.601914	112427	114235	2017-2075	1.000000	40.2
ycf1_p3	ycf1	3	TGTCGTTTCTAGTCTATCTGTTTCT	CTTTGCAGGAGAAGGCATCA	25	20	36.0	50.0	57.5	58.2	0.0	0.0	5.619845	112427	114235	2016-2074	1.000000	40.2
ycf1_p4	ycf1	4	TGTCGTTTCTAGTCTATCTGTTTCT	AGGCAGTCGCATCTCTTTTT	25	20	36.0	45.0	57.5	57.8	0.0	0.0	5.641251	112427	114235	1986-2044	1.000000	40.2
ycf1_p5	ycf1	5	TGTCGTTTCTAGTCTATCTGT	CTTTGCAGGAGAAGGCATCA	21	20	38.1	50.0	53.5	58.2	0.0	0.0	5.651834	112427	114235	2016-2074	1.000000	40.2
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	AGCTTCTCTTGCATTACCGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037669	117293	118258	1770-1770	1.000000	80.0
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	AGCTTCTCTTGCATTACCGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039039	117293	118258	1769-1769	1.000000	80.0
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	TATCTTGACGAAATGGGCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.059726	117293	118258	1841-1841	1.000000	79.8
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	TATCTTGACGAAATGGGCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.061097	117293	118258	1840-1840	1.000000	79.8
ccsA_p5	ccsA	5	TAGACACGCTGCTCTTAGGA	AGCTTCTCTTGCATTACCGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.154115	117293	118258	1760-1760	1.000000	79.0
rps19_p1	rps19	1	TTGATTCTTCGTCGCCGTAG	TGAACGATAAGCATCTATATTTTCCT	20	26	50.0	30.8	58.1	56.6	0.0	0.0	7.458595	158062	158340	425-425	1.000000	40.0
rps19_p2	rps19	2	ATTTGATTCTTCGTCGCCGT	TGAACGATAAGCATCTATATTTTCCT	20	26	45.0	30.8	58.3	56.6	0.0	0.0	7.640728	158062	158340	427-427	1.000000	40.0
rps19_p3	rps19	3	TTTGATTCTTCGTCGCCGTA	TGAACGATAAGCATCTATATTTTCCT	20	26	45.0	30.8	57.6	56.6	0.0	0.0	7.713638	158062	158340	426-426	1.000000	40.0
rps19_p4	rps19	4	TTGATTCTTCGTCGCCGTAG	TGAACGATAAGCATCTATATTTTCC	20	25	50.0	32.0	58.1	55.4	0.0	0.0	7.723629	158062	158340	425-425	1.000000	40.0
rps19_p5	rps19	5	TTGATTCTTCGTCGCCGTAG	TGAACGATAAGCATCTATATTTTCCTT	20	27	50.0	29.6	58.1	57.2	0.0	0.0	7.874597	158062	158340	425-425	1.000000	40.0