Hotspot

accD

LSC · 57070-58569 · 1500 bp

Back to Bellevalia

Pi 0.0237

MarkerSeek score 62.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1500
variable_sites: 33
pi: 0.0237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GGCTCATTTTCTATCCCGCA	AAAGAAGCCATTGCAATCGC	50.0	45.0	58.0	58.0	2122-2377 bp	1.0	85.2
accD_p2	TCTATCCCGCAATTTCAGGC	AAAGAAGCCATTGCAATCGC	50.0	45.0	58.0	58.0	2113-2368 bp	1.0	85.2
accD_p3	TGGCTCATTTTCTATCCCGC	AAAGAAGCCATTGCAATCGC	50.0	45.0	58.0	58.0	2123-2378 bp	1.0	85.2
accD_p4	GCTAAGCGCGCATAATTCAG	AAAGAAGCCATTGCAATCGC	50.0	45.0	58.1	58.0	2410-2670 bp	1.0	85.0
accD_p5	TCATCAAATAGGGGGCAGGA	AAAGAAGCCATTGCAATCGC	50.0	45.0	58.1	58.0	1976 bp	0.5	59.2