Hotspot

rpl2-trnH-GUG

IRa · 153903-153949 · 47 bp

Back to Bellevalia

Pi 0.0426
MarkerSeek score 66.00
Primer available yes
Label rpl2-trnH-GUG

Feature Metadata

label_name
rpl2-trnH-GUG
feature_type
igs
region
IRa
length_bp
47
variable_sites
2
pi
0.0426
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-trnH-GUG_p1 GTTATCCTGCGCTTGGAAGA GATTGTGAATCCACCATGCG 50.0 50.0 58.0 57.5 168 bp 1.0 76.9
rpl2-trnH-GUG_p2 TATCCTGCGCTTGGAAGAAG GATTGTGAATCCACCATGCG 50.0 50.0 57.7 57.5 166 bp 1.0 74.6
rpl2-trnH-GUG_p3 CTGCGCTTGGAAGAAGAAGT GATTGTGAATCCACCATGCG 50.0 50.0 58.5 57.5 162 bp 1.0 73.3
rpl2-trnH-GUG_p4 GTTATCCTGCGCTTGGAAGA GGGTTCAATTCCCGTCGTT 50.0 52.6 58.0 58.1 147 bp 1.0 72.4
rpl2-trnH-GUG_p5 GTTATCCTGCGCTTGGAAGA GGGTTCAATTCCCGTCGTTC 50.0 55.0 58.0 59.2 147 bp 1.0 71.3