Hotspot

rpl32-trnL-UAG

SSC · 113101-114006 · 906 bp

Back to Bellevalia

Pi 0.0234

MarkerSeek score 61.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 906
variable_sites: 21
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCAAAGTCAATTTCCACCGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1008-1017 bp	1.0	80.9
rpl32-trnL-UAG_p2	GCAAAGTCAATTTCCACCGG	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.9	57.8	1001-1010 bp	1.0	80.4
rpl32-trnL-UAG_p3	GCAAAGTCAATTTCCACCGG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.9	57.8	1007-1016 bp	1.0	80.3
rpl32-trnL-UAG_p4	GCAAAGTCAATTTCCACCGG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.9	57.8	1006-1015 bp	1.0	80.3
rpl32-trnL-UAG_p5	GCAAAGTCAATTTCCACCGG	GAGCAGCGTGTCTACCAATT	50.0	50.0	57.9	58.3	1002-1011 bp	1.0	79.7