Hotspot

psaJ-rpl33

LSC · 66785-67279 · 495 bp

Back to Bellevalia

Pi 0.0249

MarkerSeek score 63.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 11
pi: 0.0249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CCAGATGCTTTGTCATTCCC	GGGCATCTTTACCCTTAGCC	50.0	55.0	56.8	58.0	500-553 bp	1.0	75.6
psaJ-rpl33_p2	CCCAGATGCTTTGTCATTCC	GGGCATCTTTACCCTTAGCC	50.0	55.0	56.8	58.0	501-554 bp	1.0	75.6
psaJ-rpl33_p3	CCAGATGCTTTGTCATTCCCT	GGGCATCTTTACCCTTAGCC	47.6	55.0	58.3	58.0	500-553 bp	1.0	75.4
psaJ-rpl33_p4	TCCCAGATGCTTTGTCATTCC	GGGCATCTTTACCCTTAGCC	47.6	55.0	58.3	58.0	502-555 bp	1.0	75.3
psaJ-rpl33_p5	CCAGATGCTTTGTCATTCCC	CCGGCGATTCCTTATTGACA	50.0	50.0	56.8	58.1	563-616 bp	1.0	75.1