Hotspot

petA-psbJ

LSC · 62652-63404 · 753 bp

Back to Bellevalia

Pi 0.0212

MarkerSeek score 62.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 16
pi: 0.0212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTTATTTTGGCACA	GGTTCATATTCCGGGTTGGG	42.9	55.0	57.4	58.3	871-876 bp	1.0	66.2
petA-psbJ_p2	TGGCATCTGTTATTTTGGCAC	GGTTCATATTCCGGGTTGGG	42.9	55.0	57.4	58.3	872-877 bp	1.0	66.2
petA-psbJ_p3	GGCATCTGTTATTTTGGCACA	CATATTCCGGGTTGGGTTCA	42.9	50.0	57.4	57.6	867-872 bp	1.0	65.3
petA-psbJ_p4	TGGCATCTGTTATTTTGGCAC	CATATTCCGGGTTGGGTTCA	42.9	50.0	57.4	57.6	868-873 bp	1.0	65.3
petA-psbJ_p5	GGCATCTGTTATTTTGGCACA	GTTCATATTCCGGGTTGGGT	42.9	50.0	57.4	57.6	870-875 bp	1.0	65.3