Hotspot

trnF-GAA-ndhJ

LSC · 47875-48561 · 687 bp

Back to Bellevalia

Pi 0.0204

MarkerSeek score 65.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 14
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	797-821 bp	1.0	80.0
trnF-GAA-ndhJ_p2	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	840-864 bp	1.0	78.7
trnF-GAA-ndhJ_p3	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	839-863 bp	1.0	78.7
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCTTG	50.0	50.0	58.1	57.7	822-846 bp	1.0	78.2
trnF-GAA-ndhJ_p5	AAGATGATGCACGGGGAATG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.3	57.9	856-880 bp	1.0	77.8