Hotspot

trnY-GUA-trnE-UUC

LSC · 31624-31682 · 59 bp

Back to Bellevalia

Pi 0.0339

MarkerSeek score 63.30

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 2
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CGAATTTACAGTCCGTCCCC	AAGGAGAAAACGGGGATTCG	55.0	50.0	58.4	57.9	138 bp	1.0	77.5
trnY-GUA-trnE-UUC_p2	ACGAATTTACAGTCCGTCCC	AAGGAGAAAACGGGGATTCG	50.0	50.0	57.6	57.9	139 bp	1.0	77.3
trnY-GUA-trnE-UUC_p3	TGAACCAGCGTAGACATGTC	AAGGAGAAAACGGGGATTCG	50.0	50.0	57.6	57.9	163 bp	1.0	77.2
trnY-GUA-trnE-UUC_p4	CGAATTTACAGTCCGTCCCC	AAAACGGGGATTCGACTTCC	55.0	50.0	58.4	58.2	132 bp	1.0	76.9
trnY-GUA-trnE-UUC_p5	TGAACCAGCGTAGACATGTC	AAAACGGGGATTCGACTTCC	50.0	50.0	57.6	58.2	157 bp	1.0	76.6