Hotspot

trnC-GCA-petN

LSC · 28181-28918 · 738 bp

Back to Bellevalia

Pi 0.0123
MarkerSeek score 64.70
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
738
variable_sites
9
pi
0.0123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GGGGGACTGCAAATCCTTTA TTAAAGCAGCCCAAGCAAGA 50.0 45.0 57.5 58.0 819-823 bp 1.0 77.2
trnC-GCA-petN_p2 GGGGGACTGCAAATCCTTTA AGTCCACTCCTTCCCCATAC 50.0 55.0 57.5 58.1 871-875 bp 1.0 76.2
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTA GTCCACTCCTTCCCCATACT 50.0 55.0 57.5 58.1 870-874 bp 1.0 76.2
trnC-GCA-petN_p4 TTTTGGATCGAATTTGGCGG TTAAAGCAGCCCAAGCAAGA 45.0 45.0 57.4 58.0 858-862 bp 1.0 76.2
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTA TTCCCAGCTCTAGAGTCCAC 50.0 55.0 57.5 58.1 884-888 bp 1.0 76.0