Hotspot

trnG-UCC-trnR-UCU

LSC · 10223-10355 · 133 bp

Back to Bellevalia

Pi 0.0602

MarkerSeek score 75.40

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 8
pi: 0.0602
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGGGTTTCTTGATATCGCCA	50.0	45.0	58.0	56.6	294 bp	1.0	70.8
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGGGTTTCTTGATATCGCCA	55.0	45.0	58.1	56.6	297 bp	1.0	70.5
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AAGGGTTTCTTGATATCGCCA	50.0	42.9	58.0	57.4	295 bp	1.0	68.8
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AAGGGTTTCTTGATATCGCCA	55.0	42.9	58.1	57.4	298 bp	1.0	68.5
trnG-UCC-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	AGGGTTTCTTGATATCGCCA	55.0	45.0	57.7	56.6	298 bp	1.0	68.3