Hotspot

rps19

LSC · 89134-89412 · 279 bp

Back to Beilschmiedia

Pi 0.00222

MarkerSeek score 25.30

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 5
pi: 0.00222
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	AATGAACGACGAGCACCTAC	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	849-913 bp	1.0	83.7
rps19_p2	CAATGAACGACGAGCACCTA	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	850-914 bp	1.0	83.7
rps19_p3	CGATAGTACCCCCATGGACT	GAATGGTTAAAGCGCCCAAC	55.0	50.0	58.0	58.0	1173-1237 bp	1.0	83.5
rps19_p4	CAAGTCGGAAACCAAGTGGA	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	1402-1466 bp	1.0	83.2
rps19_p5	TGTACTTGTGTGCTTGTGCT	GAATGGTTAAAGCGCCCAAC	45.0	50.0	58.0	58.0	956 bp	0.052632	41.7