Hotspot

ndhF

SSC · 114950-117178 · 2229 bp

Back to Beilschmiedia

Pi 0.00549

MarkerSeek score 50.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 88
pi: 0.00549
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGAAATCCGCAACTGGTTT	GGTCTGGTGAATCGGAAACA	45.0	50.0	58.0	57.8	2398-2631 bp	0.947368	83.9
ndhF_p2	GCCCTCCATCAGTGAATCAG	GGTCTGGTGAATCGGAAACA	55.0	50.0	58.0	57.8	2420-2653 bp	0.947368	83.6
ndhF_p3	TTGGTCATTTCTTGCCCTCC	GGTCTGGTGAATCGGAAACA	50.0	50.0	58.1	57.8	2433-2666 bp	0.947368	83.3
ndhF_p4	TGTGCTTCCTGCTGATTACC	GGTCTGGTGAATCGGAAACA	50.0	50.0	57.9	57.8	2507-2740 bp	0.947368	82.9
ndhF_p5	GGATCGTGGGATTCGTTCAA	GGTCTGGTGAATCGGAAACA	50.0	50.0	58.0	57.8	2346-2383 bp	0.894737	78.5