Hotspot

ndhD

SSC · 121256-122761 · 1506 bp

Back to Beilschmiedia

Pi 0.00325

MarkerSeek score 48.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 36
pi: 0.00325
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCACAGGATTCTTTGACTTTATGTC	GCGGGTTTTTCTGGTCAAAG	36.0	50.0	57.3	57.9	1589 bp	1.0	41.9
ndhD_p2	CACAGGATTCTTTGACTTTATGTCT	GCGGGTTTTTCTGGTCAAAG	36.0	50.0	57.3	57.9	1588 bp	1.0	41.9
ndhD_p3	TCACAGGATTCTTTGACTTTATGTC	ACCGACAAAAACCCGTACTC	36.0	50.0	57.3	58.1	1626-1627 bp	1.0	41.8
ndhD_p4	CACAGGATTCTTTGACTTTATGTCT	ACCGACAAAAACCCGTACTC	36.0	50.0	57.3	58.1	1625-1626 bp	1.0	41.8
ndhD_p5	TCACAGGATTCTTTGACTTTATGT	ACCGACAAAAACCCGTACTC	33.3	50.0	56.3	58.1	1626-1627 bp	1.0	41.8