Hotspot

ndhG-ndhI

SSC · 124484-124880 · 397 bp

Back to Beilschmiedia

Pi 0.0136

MarkerSeek score 61.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 397
variable_sites: 29
pi: 0.0136
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CACCCCCAGAGCTCCTAATA	ATCAAATTGCTTTGGGTCGA	55.0	40.0	57.9	55.9	542-550 bp	0.947368	66.0
ndhG-ndhI_p2	CACCCCCAGAGCTCCTAATA	TCAAATTGCTTTGGGTCGAT	55.0	40.0	57.9	55.9	541-549 bp	0.947368	66.0
ndhG-ndhI_p3	CACCCCCAGAGCTCCTAATA	TCAAATTGCTTTGGGTCGATT	55.0	38.1	57.9	56.7	541-549 bp	0.947368	64.2
ndhG-ndhI_p4	TAATACCACCCCCAGAGCTC	ATCAAATTGCTTTGGGTCGA	55.0	40.0	58.2	55.9	552 bp	0.052632	23.9
ndhG-ndhI_p5	TAATACCACCCCCAGAGCTC	TCAAATTGCTTTGGGTCGAT	55.0	40.0	58.2	55.9	551 bp	0.052632	23.9