Hotspot

rps8-rpl14

LSC · 85670-85866 · 197 bp

Back to Beilschmiedia

Pi 0.0111

MarkerSeek score 67.30

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 13
pi: 0.0111
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GGCAATAGTGTCCCTACCCA	GCTCGGGAATTGAGACAGTT	55.0	50.0	58.8	57.9	272-285 bp	1.0	80.9
rps8-rpl14_p2	TCGGCAATAGTGTCCCTACC	GCTCGGGAATTGAGACAGTT	55.0	50.0	58.9	57.9	274-287 bp	1.0	80.1
rps8-rpl14_p3	GGCAATAGTGTCCCTACCCA	AACTCGAGTTTTTGGAGCGA	55.0	45.0	58.8	57.8	294-307 bp	1.0	79.5
rps8-rpl14_p4	TCGGCAATAGTGTCCCTACC	AACTCGAGTTTTTGGAGCGA	55.0	45.0	58.9	57.8	296-309 bp	1.0	78.8
rps8-rpl14_p5	TGTTATTCGAACCGTTCCCT	GCTCGGGAATTGAGACAGTT	45.0	50.0	56.9	57.9	332-345 bp	1.0	77.4