Hotspot

accD-psaI

LSC · 63357-64028 · 672 bp

Back to Beilschmiedia

Pi 0.00698

MarkerSeek score 68.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 32
pi: 0.00698
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTACACGGTTTCTTTCCC	TTGCAGGAAACACTAAGCCC	50.0	50.0	57.8	58.4	758-807 bp	1.0	83.0
accD-psaI_p2	GCTACACGGTTTCTTTCCCT	TTGCAGGAAACACTAAGCCC	50.0	50.0	57.8	58.4	757-806 bp	1.0	83.0
accD-psaI_p3	AGCTACACGGTTTCTTTCCC	CAGGAAACACTAAGCCCACT	50.0	50.0	57.8	57.4	755-804 bp	1.0	81.5
accD-psaI_p4	GCTACACGGTTTCTTTCCCT	CAGGAAACACTAAGCCCACT	50.0	50.0	57.8	57.4	754-803 bp	1.0	81.5
accD-psaI_p5	ATTCAAGTAGAGCGCTAGGC	TTGCAGGAAACACTAAGCCC	50.0	50.0	57.5	58.4	728-777 bp	1.0	80.4