Hotspot

rpoB-trnC-GCA

LSC · 28819-29989 · 1171 bp

Back to Beilschmiedia

Pi 0.00331

MarkerSeek score 63.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1171
variable_sites: 30
pi: 0.00331
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCGTCAAACCTTGGTCAATGA	CCGGATTTGAACTGGGGAAA	42.9	50.0	57.8	58.1	1289-1306 bp	1.0	72.9
rpoB-trnC-GCA_p2	TCTTTCGTCAAACCTTGGTCA	CCGGATTTGAACTGGGGAAA	42.9	50.0	57.7	58.1	1293-1310 bp	1.0	72.4
rpoB-trnC-GCA_p3	TCGTCAAACCTTGGTCAATGA	TTGTTAATCAGGCGACACCC	42.9	50.0	57.8	58.2	1307-1324 bp	1.0	72.1
rpoB-trnC-GCA_p4	TCTTTCGTCAAACCTTGGTCA	TTGTTAATCAGGCGACACCC	42.9	50.0	57.7	58.2	1311-1328 bp	1.0	71.5
rpoB-trnC-GCA_p5	TCGTCAAACCTTGGTCAATGA	AAAAGGATTTGCAGTCCCCC	42.9	50.0	57.8	58.4	1271-1288 bp	1.0	70.8