Hotspot

trnH-GUG-psbA

LSC · 1634-2021 · 388 bp

Back to Beilschmiedia

Pi 0.0104

MarkerSeek score 47.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 19
pi: 0.0104
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	52.6	55.0	56.9	57.9	445-454 bp	1.0	68.0
trnH-GUG-psbA_p2	ATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	52.6	55.0	56.9	57.9	448-457 bp	1.0	67.9
trnH-GUG-psbA_p3	ATCCACTTGGCTACATCCG	TAGACCTAGCTGCTGTCGAA	52.6	50.0	56.9	57.9	441-450 bp	1.0	67.6
trnH-GUG-psbA_p4	ATCCACTTGGCTACATCCGC	CCTCTAGACCTAGCTGCTGT	55.0	55.0	60.2	57.9	445-454 bp	1.0	67.3
trnH-GUG-psbA_p5	CCACTTGGCTACATCCGC	CCTCTAGACCTAGCTGCTGT	61.1	55.0	58.2	57.9	443-452 bp	1.0	67.3