Hotspot

rpl32-trnL-UAG

SSC · 117455-118574 · 1120 bp

Back to Begonia

Pi 0.0582
MarkerSeek score 80.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1120
variable_sites
591
pi
0.0582
unique_haplotype_count
106
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAGCAAAGGGTATTGGGCA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 761-1311 bp 0.884956 87.2
rpl32-trnL-UAG_p2 AAAGCAAAGGGTATTGGGCA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 762-1312 bp 0.884956 87.1
rpl32-trnL-UAG_p3 AAAGCAAAGGGTATTGGGCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.0 57.9 771-1321 bp 0.884956 86.2
rpl32-trnL-UAG_p4 CGGCGAAAATAGACAATGCC AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1100-1661 bp 0.787611 82.6
rpl32-trnL-UAG_p5 CGGCGAAAATAGACAATGCC CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 1101-1662 bp 0.787611 82.6