Hotspot

ndhF-rpl32

SSC · 116315-117295 · 981 bp

Back to Begonia

Pi 0.0752

MarkerSeek score 82.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 981
variable_sites: 551
pi: 0.0752
unique_haplotype_count: 107
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGCATTCGACGCAATAGGT	TGCCCAATACCCTTTGCTTT	45.0	45.0	58.0	58.0	988-1626 bp	0.769912	82.4
ndhF-rpl32_p2	AAGCATTCGACGCAATAGGT	TGTTTAGGGTCGATGGGGAT	45.0	50.0	58.0	58.1	1401-2051 bp	0.752212	81.0
ndhF-rpl32_p3	ACCACACATGAAGCGGAAAT	TGCCCAATACCCTTTGCTTT	45.0	45.0	58.1	58.0	1213-1851 bp	0.725664	80.1
ndhF-rpl32_p4	TTTTGACAAGCATTCGACGC	TGTTTAGGGTCGATGGGGAT	45.0	50.0	58.0	58.1	1408-2058 bp	0.725664	79.9
ndhF-rpl32_p5	TTTTGACAAGCATTCGACGC	TGCCCAATACCCTTTGCTTT	45.0	45.0	58.0	58.0	995-1633 bp	0.646018	77.4