Hotspot

ycf1-ndhF

SSC · 114088-115114 · 1027 bp

Back to Begonia

Pi 0.0401

MarkerSeek score 78.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 1027
variable_sites: 387
pi: 0.0401
unique_haplotype_count: 106
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACAGGAACAAGAGGGATCCA	GGTTACCTGATGCCATGGAG	50.0	55.0	58.0	58.0	1873-2128 bp	0.982301	90.7
ycf1-ndhF_p2	ACAGGAACAAGAGGGATCCA	GTTACCTGATGCCATGGAGG	50.0	55.0	58.0	58.0	1872-2127 bp	0.982301	90.7
ycf1-ndhF_p3	AAGAGGGATCCACCGAAGAA	GTTACCTGATGCCATGGAGG	50.0	55.0	58.0	58.0	1864-2119 bp	0.982301	90.7
ycf1-ndhF_p4	ACAGGAACAAGAGGGATCCA	ACCTATTGCGTCGAATGCTT	50.0	45.0	58.0	58.0	2115-2370 bp	0.955752	89.7
ycf1-ndhF_p5	AAGAGGGATCCACCGAAGAA	ACCTATTGCGTCGAATGCTT	50.0	45.0	58.0	58.0	2107-2362 bp	0.955752	89.7