Hotspot

psbE-petL

LSC · 56543-57832 · 1290 bp

Back to Begonia

Pi 0.0404

MarkerSeek score 72.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1290
variable_sites: 584
pi: 0.0404
unique_haplotype_count: 110
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1380-2096 bp	1.0	91.7
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1379-2095 bp	1.0	91.7
psbE-petL_p3	ACGAGGGCTTCCAAAAACAT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1315-2031 bp	1.0	91.7
psbE-petL_p4	AGGGCTTCCAAAAACATCGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1312-2028 bp	0.99115	91.3
psbE-petL_p5	AGGGCTTCCAAAAACATCGT	CCTACAGAGCGTGATTGCAT	45.0	50.0	58.0	58.1	1272-1988 bp	0.929204	88.7