Hotspot

trnT-GGU-psbD

LSC · 22825-24371 · 1547 bp

Back to Begonia

Pi 0.0345
MarkerSeek score 80.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1547
variable_sites
594
pi
0.0345
unique_haplotype_count
110
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG TCCCTCCGTAACCAGTCATC 55.0 55.0 58.1 58.5 1373-1762 bp 0.982301 86.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA TCCCTCCGTAACCAGTCATC 50.0 55.0 58.1 58.5 1376-1765 bp 0.973451 86.1
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA TCCCTCCGTAACCAGTCATC 50.0 55.0 58.3 58.5 1350-1739 bp 1.0 85.6
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG GTCCCTCCGTAACCAGTCAT 55.0 55.0 58.1 58.8 1374-1763 bp 0.982301 84.3
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG CCTCCGTAACCAGTCATCCA 55.0 55.0 58.1 58.8 1371-1760 bp 0.982301 84.3