Hotspot

trnD-GUC-trnY-GUA

LSC · 20862-21340 · 479 bp

Back to Begonia

Pi 0.0441

MarkerSeek score 78.10

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 201
pi: 0.0441
unique_haplotype_count: 105
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	ATTCTTCTTGGGTCGATGCC	50.0	50.0	58.5	58.0	387-635 bp	0.982301	87.1
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	334-582 bp	0.982301	85.6
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	333-581 bp	0.982301	85.5
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	355-603 bp	0.973451	84.3
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	354-602 bp	0.973451	84.1