Hotspot

psbM-trnD-GUC

LSC · 19880-20784 · 905 bp

Back to Begonia

Pi 0.0431

MarkerSeek score 79.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 364
pi: 0.0431
unique_haplotype_count: 104
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGAACTAGAATGAACAGTGT	GTTCGAGCCCCGTCAGTC	38.1	66.7	54.2	60.1	759-1007 bp	1.0	52.0
psbM-trnD-GUC_p2	GCAGTAGGAACTAGAATGAACAGT	GTTCGAGCCCCGTCAGTC	41.7	66.7	58.3	60.1	764-1012 bp	0.982301	51.3
psbM-trnD-GUC_p3	GCAGTAGGAACTAGAATGAACAG	GTTCGAGCCCCGTCAGTC	43.5	66.7	56.7	60.1	764-1012 bp	0.982301	51.3
psbM-trnD-GUC_p4	GCAGTAGGAACTAGAATGAACA	GTTCGAGCCCCGTCAGTC	40.9	66.7	55.6	60.1	764-1012 bp	0.982301	51.3
psbM-trnD-GUC_p5	AGAATGAACAGTGTAGTAGCA	GTTCGAGCCCCGTCAGTC	38.1	66.7	54.3	60.1	752-1000 bp	0.964602	50.6