Hotspot

trnC-GCA-petN

LSC · 17973-18715 · 743 bp

Back to Begonia

Pi 0.0414

MarkerSeek score 80.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 329
pi: 0.0414
unique_haplotype_count: 103
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	503-1027 bp	1.0	88.5
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	442-966 bp	0.955752	88.4
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	521-1045 bp	1.0	86.3
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	460-984 bp	0.955752	86.2
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	441-965 bp	0.955752	82.4