Hotspot

rpoB-trnC-GCA

LSC · 16750-17901 · 1152 bp

Back to Begonia

Pi 0.0442

MarkerSeek score 79.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1152
variable_sites: 470
pi: 0.0442
unique_haplotype_count: 110
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1341-1874 bp	0.973451	90.1
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	TCTTCCACTTGCTTCTGCTG	50.0	50.0	58.0	58.1	1432-1965 bp	0.778761	82.0
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	TTCAGGCACAACACCATTCA	50.0	45.0	58.0	57.9	2000-2492 bp	0.504425	71.4
rpoB-trnC-GCA_p4	CGAGCCCATATCCTTGCTTT	ATTCCATTCGTGACTGTGGG	50.0	50.0	58.0	57.9	1786-2211 bp	0.141593	56.4
rpoB-trnC-GCA_p5	CGAGCCCATATCCTTGCTTT	GCAGCAGCCACTACATTAGT	50.0	50.0	58.0	58.0	1605-2059 bp	0.097345	54.4