Hotspot

rps19-rpl2

IRb · 87470-87541 · 72 bp

Back to Beesia

Pi 0.123
MarkerSeek score 87.50
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
IRb
length_bp
72
variable_sites
7
pi
0.123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.79
markerseek_score
87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 CGGGACCAAGTTACGATTGT GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 244-259 bp 1.0 85.3
rps19-rpl2_p2 CGGGACCAAGTTACGATTGT TTGATTCTTCGTCGCCGTAG 50.0 50.0 57.9 58.1 191-206 bp 1.0 85.3
rps19-rpl2_p3 CGGGACCAAGTTACGATTGT GGGCCCCAATTGGTAGAAAA 50.0 50.0 57.9 58.1 283-298 bp 1.0 84.8
rps19-rpl2_p4 CGGGACCAAGTTACGATTGT CACAACCCCTTGGGGTTATC 50.0 55.0 57.9 58.2 258-273 bp 1.0 84.2
rps19-rpl2_p5 CGGGACCAAGTTACGATTGT TATCCTGCGCTTGGAAGAAG 50.0 50.0 57.9 57.7 242-257 bp 1.0 83.0