Hotspot

petD-rpoA

LSC · 80878-81089 · 212 bp

Back to Beesia

Pi 0.033
MarkerSeek score 71.50
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
212
variable_sites
7
pi
0.033
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 TTGATTGGTACCGCAGTAGC TGCAAATTGAGCATTTTCGCA 50.0 38.1 58.0 58.2 397 bp 1.0 71.3
petD-rpoA_p2 AGCCCTTTGGTTGGGTATTG TGCAAATTGAGCATTTTCGCA 50.0 38.1 58.1 58.2 380 bp 1.0 71.0
petD-rpoA_p3 GGTTGGGTATTGGAGCAACA TGCAAATTGAGCATTTTCGCA 50.0 38.1 58.1 58.2 372 bp 1.0 70.9
petD-rpoA_p4 TGGTTGGGTATTGGAGCAAC TGCAAATTGAGCATTTTCGCA 50.0 38.1 58.1 58.2 373 bp 1.0 70.9
petD-rpoA_p5 AGTAGCCCTTTGGTTGGGTA TGCAAATTGAGCATTTTCGCA 50.0 38.1 58.2 58.2 383 bp 1.0 69.5