Hotspot

psbE-petL

LSC · 67850-68890 · 1041 bp

Back to Beesia

Pi 0.0156
MarkerSeek score 56.90
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1041
variable_sites
16
pi
0.0156
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA CCAGTAGAAAACCGAAATAACT 50.0 36.4 57.9 54.1 1175-1182 bp 1.0 42.1
psbE-petL_p2 ACGAATAACCAACCCGCAAT CCAGTAGAAAACCGAAATAACT 45.0 36.4 58.2 54.1 1184-1191 bp 1.0 42.1
psbE-petL_p3 TGCTGACGAATAACCAACCC CCAGTAGAAAACCGAAATAACT 50.0 36.4 58.2 54.1 1189-1196 bp 1.0 42.1
psbE-petL_p4 CGAATAACCAACCCGCAATG CCAGTAGAAAACCGAAATAACT 50.0 36.4 57.8 54.1 1183-1190 bp 1.0 42.1
psbE-petL_p5 ACCAACCCGCAATGAATAGG CCAGTAGAAAACCGAAATAACT 50.0 36.4 58.2 54.1 1177-1184 bp 1.0 42.1