Hotspot

ndhC-trnV-UAC

LSC · 53950-55117 · 1168 bp

Back to Beesia

Pi 0.0282
MarkerSeek score 67.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1168
variable_sites
31
pi
0.0282
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 36.4 50.0 56.1 57.8 1195-1224 bp 1.0 53.4
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 36.4 55.0 56.1 57.5 1194-1223 bp 1.0 50.6
ndhC-trnV-UAC_p3 TCATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 36.4 55.0 56.1 58.7 1183-1212 bp 1.0 49.5
ndhC-trnV-UAC_p4 CATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 38.1 50.0 54.6 57.8 1194-1223 bp 1.0 49.4
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 34.8 50.0 56.6 57.8 1195-1224 bp 1.0 49.3