Hotspot

trnC-GCA-petN

LSC · 29195-30059 · 865 bp

Back to Beesia

Pi 0.0173
MarkerSeek score 61.10
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
865
variable_sites
15
pi
0.0173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.1 58.0 928-929 bp 1.0 80.6
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACT 50.0 50.0 58.1 57.8 922-923 bp 1.0 79.1
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.4 58.0 946-947 bp 1.0 78.4
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 982-983 bp 1.0 77.8
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT CAGCCCAAGCAAGACTTACT 50.0 50.0 58.4 57.8 940-941 bp 1.0 76.9