Hotspot

rps16-trnQ-UUG

LSC · 6242-8040 · 1799 bp

Back to Beesia

Pi 0.0205
MarkerSeek score 60.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1799
variable_sites
28
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.76
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ACCGATCATGTCCTTCAAGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1468-1888 bp 1.0 84.5
rps16-trnQ-UUG_p2 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1473-1893 bp 1.0 83.8
rps16-trnQ-UUG_p3 ACCGATCATGTCCTTCAAGC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 1472-1892 bp 1.0 83.4
rps16-trnQ-UUG_p4 CCACAACCGATCATGTCCTT CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 1477-1897 bp 1.0 82.7
rps16-trnQ-UUG_p5 ATCCACAACCGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 1475-1895 bp 1.0 82.1