Hotspot

ycf1

SSC · 123241-126750 · 3510 bp

Back to Beccarinda

Pi 0.0168

MarkerSeek score 52.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3510
variable_sites: 59
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTTGTGGGAGTTTGGTGGA	GAAAGGCTGGTTGAAAGGGT	50.0	50.0	57.9	58.3	650 bp	1.0	79.6
ycf1_p2	CTTTGTGGGAGTTTGGTGGA	TGGAAAGGCTGGTTGAAAGG	50.0	50.0	57.9	58.3	652 bp	1.0	79.6
ycf1_p3	TGGGAAAGAATGGAGCGAAG	GAAAGGCTGGTTGAAAGGGT	50.0	50.0	57.9	58.3	945 bp	1.0	79.0
ycf1_p4	TGTGGGAGTTTGGTGGATTG	GAAAGGCTGGTTGAAAGGGT	50.0	50.0	58.0	58.3	647 bp	0.5	57.6
ycf1_p5	TGTGGGAGTTTGGTGGATTG	TGGAAAGGCTGGTTGAAAGG	50.0	50.0	58.0	58.3	649 bp	0.5	57.6