Hotspot

ndhI

SSC · 118612-119103 · 492 bp

Back to Beccarinda

Pi 0.0122

MarkerSeek score 51.10

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 492
variable_sites: 6
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GGTTTTTGAATTAGGGATTTTATACA	TCAAACAAGAGAAAGAAACAAGT	26.9	30.4	54.5	54.9	574 bp	1.0	40.8
ndhI_p2	GGTTTTTGAATTAGGGATTTTATACA	CTTTCACTGTAAAAGAATTCCCT	26.9	34.8	54.5	54.8	611 bp	1.0	40.8
ndhI_p3	GGTTTTTGAATTAGGGATTTTATACA	CACTGTAAAAGAATTCCCTATTCACA	26.9	34.6	54.5	57.7	607 bp	1.0	40.8
ndhI_p4	GGTTTTTGAATTAGGGATTTTATACA	TTCACTGTAAAAGAATTCCCT	26.9	33.3	54.5	52.6	609 bp	1.0	40.8
ndhI_p5	GGTTTTTGAATTAGGGATTTTATACA	TTTCACTGTAAAAGAATTCCCT	26.9	31.8	54.5	53.5	610 bp	1.0	40.8