Hotspot

ycf1-trnN-GUU

IRa · 126751-128935 · 2185 bp

Back to Beccarinda

Pi 0.017

MarkerSeek score 55.70

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 2185
variable_sites: 37
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ACCTCGCCGAAAATCTGATT	AGGATTCGAACCTACGACCA	45.0	50.0	57.6	58.2	2277-2283 bp	1.0	77.2
ycf1-trnN-GUU_p2	ACCTCGCCGAAAATCTGATT	CCCCAAGTAGGATTCGAACC	45.0	55.0	57.6	57.7	2285-2291 bp	1.0	75.9
ycf1-trnN-GUU_p3	CCTCGCCGAAAATCTGATTG	AGGATTCGAACCTACGACCA	50.0	50.0	57.3	58.2	2276-2282 bp	1.0	74.5
ycf1-trnN-GUU_p4	ACCTCGCCGAAAATCTGATT	GGATTCGAACCTACGACCAA	45.0	50.0	57.6	57.3	2276-2282 bp	1.0	73.2
ycf1-trnN-GUU_p5	CCTCGCCGAAAATCTGATTG	CCCCAAGTAGGATTCGAACC	50.0	55.0	57.3	57.7	2284-2290 bp	1.0	73.1