Hotspot

ccsA-ndhD

SSC · 114830-115075 · 246 bp

Back to Beccarinda

Pi 0.0717
MarkerSeek score 76.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
246
variable_sites
16
pi
0.0717
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGGGTCAATCTATTAGGAATAGG ACCCGTACTAAGTATTGGTATGT 41.7 39.1 57.0 56.5 381-404 bp 1.0 47.3
ccsA-ndhD_p2 GGGGTCAATCTATTAGGAATAGGAC ACCCGTACTAAGTATTGGTATGT 44.0 39.1 58.1 56.5 380-403 bp 1.0 47.3
ccsA-ndhD_p3 GGGGTCAATCTATTAGGAATAGGA ACCCGTACTAAGTATTGGTATGT 41.7 39.1 56.8 56.5 380-403 bp 1.0 47.3
ccsA-ndhD_p4 TTGGGGTCAATCTATTAGGAATAGG ACCCGTACTAAGTATTGGTATGT 40.0 39.1 57.7 56.5 382-405 bp 1.0 47.3
ccsA-ndhD_p5 TGGGGTCAATCTATTAGGAA ACCCGTACTAAGTATTGGTATGT 40.0 39.1 52.6 56.5 381-404 bp 1.0 47.3