Hotspot

psbN-psbH

LSC · 75049-75135 · 87 bp

Back to Beccarinda

Pi 0.023

MarkerSeek score 65.70

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 87
variable_sites: 2
pi: 0.023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	TGTTGAGAAGGTTGCCCAAA	AACAGTAGTTCGTCTTGGGC	45.0	50.0	57.8	57.8	260 bp	1.0	78.1
psbN-psbH_p2	TTGTTGAGAAGGTTGCCCAA	AACAGTAGTTCGTCTTGGGC	45.0	50.0	57.8	57.8	261 bp	1.0	78.1
psbN-psbH_p3	TGTTGAGAAGGTTGCCCAAA	GGCCCGATCTAGAACTGTTC	45.0	55.0	57.8	57.8	243 bp	1.0	77.6
psbN-psbH_p4	TTGTTGAGAAGGTTGCCCAA	GGCCCGATCTAGAACTGTTC	45.0	55.0	57.8	57.8	244 bp	1.0	77.6
psbN-psbH_p5	AGTTGTTGAGAAGGTTGCCC	AACAGTAGTTCGTCTTGGGC	50.0	50.0	58.3	57.8	263 bp	1.0	76.8