Hotspot

cemA-petA

LSC · 62442-62782 · 341 bp

Back to Beccarinda

Pi 0.0294

MarkerSeek score 56.30

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 10
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	CTCTTGGGCTCAGGAATTCC	ATAACCCTGCTGCCCAAAAA	55.0	45.0	58.0	58.0	627 bp	1.0	82.7
cemA-petA_p2	CTCTTGGGCTCAGGAATTCC	CTTGCTTCCGTCGGGATAAA	55.0	50.0	58.0	58.0	1095 bp	1.0	81.7
cemA-petA_p3	CTCTTGGGCTCAGGAATTCC	TGTTACTCTTGCTTCCGTCG	55.0	50.0	58.0	57.9	1102 bp	1.0	81.4
cemA-petA_p4	CTCTTGGGCTCAGGAATTCC	TAACCCTGCTGCCCAAAAAT	55.0	45.0	58.0	58.0	626 bp	0.5	59.4
cemA-petA_p5	CTCTTGGGCTCAGGAATTCC	TCCGACGGATCTGTTATGGA	55.0	50.0	58.0	57.9	1196 bp	0.5	59.1