Hotspot

ycf4-cemA

LSC · 61012-61880 · 869 bp

Back to Beccarinda

Pi 0.028

MarkerSeek score 73.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 869
variable_sites: 23
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTGACCCGTACTGATGAG	GAGAGAGAAATCCAACAGGACA	55.0	45.5	58.1	57.5	998-1044 bp	1.0	64.4
ycf4-cemA_p2	CGTTGACCCGTACTGATGAG	TGAGAGAGAAATCCAACAGGAC	55.0	45.5	58.1	57.5	999-1045 bp	1.0	64.4
ycf4-cemA_p3	ACAAAAAGCCGCTGAATTGG	GAGAGAGAAATCCAACAGGACA	45.0	45.5	57.8	57.5	955-1001 bp	1.0	64.2
ycf4-cemA_p4	ACAAAAAGCCGCTGAATTGG	TGAGAGAGAAATCCAACAGGAC	45.0	45.5	57.8	57.5	956-1002 bp	1.0	64.1
ycf4-cemA_p5	CGTTGACCCGTACTGATGAG	TCCAACAGGACAAAAACACT	55.0	40.0	58.1	55.3	988-1034 bp	1.0	63.3