Hotspot

accD-psaI

LSC · 59184-59922 · 739 bp

Back to Beccarinda

Pi 0.0207

MarkerSeek score 68.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 739
variable_sites: 15
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TAAGCTCCACGCCTTTTTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.5	58.0	821-834 bp	1.0	78.8
accD-psaI_p2	TAAGCTCCACGCCTTTTTCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.5	57.9	827-840 bp	1.0	78.2
accD-psaI_p3	GGTTCACAAGCGGCTGAATA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.6	58.0	913-926 bp	1.0	77.9
accD-psaI_p4	GGTTCACAAGCGGCTGAATA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.6	57.9	919-932 bp	1.0	77.3
accD-psaI_p5	TAAGCTCCACGCCTTTTTCC	GCCGGAAATACTAGGCCTAC	50.0	55.0	58.5	57.5	810-823 bp	1.0	75.5